Journal: Life
Article Title: Modulation of Oxidative and ER Stress Pathways by the ADAM17 Inhibitor GW280264X in LPS-Induced Acute Liver Injury
doi: 10.3390/life15121877
Figure Lengend Snippet: Effects of GW280264X on redox regulatory proteins in liver tissue. ( A ) Kelch-like ECH-associated protein 1 (Keap1), ( B ) nuclear factor erythroid 2 2-related factor 2 (Nrf2), and ( C ) glutathione (GSH) levels. Data are presented as mean ± SEM ( n = 5). * p < 0.05 vs. 0.9% NaCl, # p < 0.05 vs. LPS. Black squares represent individual data points for each animal.
Article Snippet: Commercial ELISA kits were used to quantify liver tissue levels of KEAP1 (Cat. No. E2198Mo, BT-Laboratory, Shanghai, China), CHOP (Cat. No. E2718Mo, BT-Laboratory), 4-HNE (Cat. No. E1293Mo, BT-Laboratory), MDA (Cat. No. E0625Mo, BT-Laboratory), NRF2 (Cat. No. E1367Mo, BT-Laboratory), TNF-α (Cat. No. E-EL-M3063, Elabscience, Houston, TX, USA), GRP78 (Cat. No. E-EL-M2696, Elabscience, Houston, TX, USA), glutathione (GSH) colorimetric assay kit (Cat No. E-EL-0026, Elabscience, Houston, TX, USA) and ATF6 (Cat. No. ELK4479, Elk Biotechnology, Wuhan, China).
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